CONCURRENT OPTIMIZATION IN SELECTION OF PRIMER AND

专利名称：CONCURRENT OPTIMIZATION IN

SELECTION OF PRIMER AND CAPTUREPROBE SETS FOR NUCLEIC ACID ANALYSIS

发明人：Michael Seul,Tatiana Vener,Xiongwu Xia申请号：US13169201申请日：20110627

公开号：US20120190585A1公开日：20120726

摘要：Disclosed is a method of iteratively optimizing two (or more) interrelated setsof probes for the multi-step analysis of sets of designated sequences, each suchsequence requiring, for conversion, at least one conversion probe (“primer”), and eachconverted sequence requiring, for detection, at least one capture probe. The iterativemethod disclosed herein for the concurrent optimization of primer and probe selectioninvokes fast logical string matching functions to perform a complete cross-correlation ofprobe sequences and target sequences. The score function assigns to each probe-targetalignment a “degree of matching” score on the basis of position-weighted Hammingdistance functions introduced herein. Pairs of probes in the final selection may differ inseveral positions, while other pairs of probes may differ in only a single position. Not allsuch positions are of equal importance, and a score function is introduced, reflecting theposition of the mismatch within the probe sequence.

申请人：Michael Seul,Tatiana Vener,Xiongwu Xia

地址：Fanwood NJ US,Stirling NJ US,Irvine CA US

国籍：US,US,US

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