您好,欢迎来到华佗健康网。
搜索
您的当前位置:首页CONCURRENT OPTIMIZATION IN SELECTION OF PRIMER AND

CONCURRENT OPTIMIZATION IN SELECTION OF PRIMER AND

来源:华佗健康网
专利内容由知识产权出版社提供

专利名称:CONCURRENT OPTIMIZATION IN

SELECTION OF PRIMER AND CAPTUREPROBE SETS FOR NUCLEIC ACID ANALYSIS

发明人:Michael Seul,Tatiana Vener,Xiongwu Xia申请号:US13169201申请日:20110627

公开号:US20120190585A1公开日:20120726

摘要:Disclosed is a method of iteratively optimizing two (or more) interrelated setsof probes for the multi-step analysis of sets of designated sequences, each suchsequence requiring, for conversion, at least one conversion probe (“primer”), and eachconverted sequence requiring, for detection, at least one capture probe. The iterativemethod disclosed herein for the concurrent optimization of primer and probe selectioninvokes fast logical string matching functions to perform a complete cross-correlation ofprobe sequences and target sequences. The score function assigns to each probe-targetalignment a “degree of matching” score on the basis of position-weighted Hammingdistance functions introduced herein. Pairs of probes in the final selection may differ inseveral positions, while other pairs of probes may differ in only a single position. Not allsuch positions are of equal importance, and a score function is introduced, reflecting theposition of the mismatch within the probe sequence.

申请人:Michael Seul,Tatiana Vener,Xiongwu Xia

地址:Fanwood NJ US,Stirling NJ US,Irvine CA US

国籍:US,US,US

更多信息请下载全文后查看

因篇幅问题不能全部显示,请点此查看更多更全内容

Copyright © 2019- huatuo0.com 版权所有 湘ICP备2023021991号-1

违法及侵权请联系:TEL:199 1889 7713 E-MAIL:2724546146@qq.com

本站由北京市万商天勤律师事务所王兴未律师提供法律服务