17-Hydroxyprogesterone_LCMS_17150_MedChemExpress

Data File D:\\AGLIENT 1260\\DATA\\20150724\\20150724 2015-07-24 09-35-46\\BIZ2015-724-DJL3.DSample Name: BIZ2015-724-DJL3=====================================================================Acq. Operator : Li Shan(LCMS-02) Seq. Line : 14Acq. Instrument : HY-LCMS-02 Location : P1-B-03Injection Date : 7/24/2015 10:32:39 AM Inj : 1 Inj Volume : 3.000 µlAcq. Method : D:\\AGLIENT 1260\\DATA\\20150724\\20150724 2015-07-24 09-35-46\\100-1000MS+3MIN- 1.5_(0.02%FA).MLast changed : 7/24/2015 9:35:47 AM by Li Shan(LCMS-02)Analysis Method : D:\\AGLIENT 1260\\DATA\\20150722\\20150722 2015-07-22 10-02-30\\100-1000MS+3MIN- 1.5_(0.02%FA).M (Sequence Method)Last changed : 7/24/2015 1:45:46 PM by Li Shan(LCMS-02) (modified after loading)Method Info : Postive,MS:100-1000,Column ID:A-RP-132,40℃ Catalog No : HY-B01 Batch#17150 A-RP-134 Additional Info : Peak(s) manually integrated DAD1 C, Sig=2,4 Ref=off (D:\\AGLIENT...60\\DATA\\20150724\\20150724 2015-07-24 09-35-46\\BIZ2015-724-DJL3.D) 2.360 1.982 2.163 2.0mAU1200100080060040020000.511.522.53min ===================================================================== Area Percent Report ===================================================================== Sorted By : SignalMultiplier : 1.0000Dilution : 1.0000Do not use Multiplier & Dilution Factor with ISTDs Signal 1: DAD1 C, Sig=2,4 Ref=off Peak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] %----|-------|----|-------|----------|----------|--------| 1 1.982 MM 0.0514 3.01278 9.77429e-1 0.0860 2 2.163 MM 0.0712 2.47207 5.787e-1 0.0706 3 2.360 MF 0.0439 3488.52319 1325.673 99.5944 4 2.0 FM 0.0623 8.72052 2.33344 0.2490 Totals : 3502.72856 1329.78658 ===================================================================== *** End of Report ***HY-LCMS-02 7/24/2015 1:45:47 PM Li Shan(LCMS-02)Page 1 of 1

Data File D:\\AGLIENT 1260\\DATA\\20150724\\20150724 2015-07-24 09-35-46\\BIZ2015-724-DJL3.DSample Name: BIZ2015-724-DJL3=====================================================================Acq. Operator : Li Shan(LCMS-02) Seq. Line : 14Acq. Instrument : HY-LCMS-02 Location : P1-B-03Injection Date : 7/24/2015 10:32:39 AM Inj : 1 Inj Volume : 3.000 µlAcq. Method : D:\\AGLIENT 1260\\DATA\\20150724\\20150724 2015-07-24 09-35-46\\100-1000MS+3MIN- 1.5_(0.02%FA).MLast changed : 7/24/2015 9:35:47 AM by Li Shan(LCMS-02)Analysis Method : D:\\AGLIENT 1260\\DATA\\20150722\\20150722 2015-07-22 10-02-30\\100-1000MS+3MIN- 1.5_(0.02%FA).M (Sequence Method)Last changed : 7/24/2015 1:46:52 PM by Li Shan(LCMS-02) (modified after loading)Method Info : Postive,MS:100-1000,Column ID:A-RP-132,40℃ Catalog No : HY-B01 Batch#17150 A-RP-134 Additional Info : Peak(s) manually integrated MSD1 TIC, MS File (D:\\AGLIENT 1260\\DATA\\20150724\\20150724 2015-07-24 09-35-46\\BIZ2015-724-DJL3.D) ES-API, Pos, Sca 2.3624000003500003000002500002000001500001000005000000.511.522.53min HY-LCMS-02 7/24/2015 1:46: PM Li Shan(LCMS-02)Page 1 of 2

Data File D:\\AGLIENT 1260\\DATA\\20150724\\20150724 2015-07-24 09-35-46\\BIZ2015-724-DJL3.DSample Name: BIZ2015-724-DJL3MS Signal: MSD1 TIC, MS File, ES-API, Pos, Scan, Frag: 50 Spectra averaged over upper half of peaks. Noise Cutoff: 1000 counts. Reportable Ion Abundance: > 10%. Retention Mol. WeightTime (MS) MS Area or Ion 2.362 1778538 394.20 I 332.20 I 331.20 I*MSD1 SPC, time=2.345:2.382 of D:\\AGLIENT 1260\\DATA\\20150724\\20150724 2015-07-24 09-35-46\\BIZ2015-724-DJL3.D ES-API,100806040200100200300400500600700800m/z 331.2Max: 205973 332.2 *** End of Report ***HY-LCMS-02 7/24/2015 1:46: PM Li Shan(LCMS-02) 372.2 394.2Page 2 of 2