Methods and compositions for generation of multipl

专利名称：Methods and compositions for generation

of multiple copies of nucleic acid sequencesand methods of detection thereof

发明人：Nurith Kurn申请号：US10017880申请日：20011213

公开号：US20030186234A1公开日：20031002

摘要：The present invention provides novel isothermal methods of generatingmultiple copies of, detecting and/or quantifying nucleic acid sequences of interest basedon limited primer extension or attachment of oligonucleotide pairs using compositeRNA/DNA primers. Methods for generating multiple copies of and/or detecting and/orquantifying nucleic acid sequences, wherein products of primer extension or attachmentof oligonucleotide pairs comprising a cleavable portion are generated, and whereincleavage of the products results in dissociation of cleaved products from targetpolynucleotides, are provided. The invention further provides compositions, kits andsystems for practicing these methods.

申请人：KURN NURITH

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