METHOD FOR THE COLORIMETRIC DETECTION OF CONTAMINA

专利名称：METHOD FOR THE COLORIMETRIC

DETECTION OF CONTAMINATION WITHNUCLEASES

发明人：POMPA, Pier Paolo,VALENTINI,

Paola,CECERE, Paola

申请号：EP15823781.8申请日：20151211公开号：EP3234176A1公开日：20171025

摘要：Method for detecting the presence of nucleases in a sample, characterized inthat it comprises the steps of: —incubating the sample to be tested for the presence ofnucleases with at least one oligonucleotide linker constituting the substrate for thenuclease to be detected, for a sufficient time to cause degradation of said

oligonucleotide linker by the nuclease possibly present in the sample, —adding to thesample, upon incubation, colloidal gold nanoparticles comprising gold nanoparticlesfunctionalized with a first probe oligonucleotide and gold nanoparticles functionalizedwith a respective second probe oligonucleotide, said first and second probeoligonucleotides being complementary to a respective portion of the nucleotidesequence of the oligonucleotide linker, and—examining the possible colour change ofthe sample as a result of the addition of said nanoparticles, a colour change of thesample to the colour assumed by the colloidal gold particles when aggregated at adistance less than their size being indicative of the absence of the tested nuclease fromthe sample.

申请人：Fondazione Istituto Italiano di Tecnologia

地址：Via Morego 30 16163 Genova IT

国籍：IT

代理机构：Comoglio, Elena

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